学長　木原 裕

経歴

1967年

東京大学理学部卒業

1972年

東京大学理学研究科単位取得退学

1979年

理学博士(東京大学)

学会

日本バイオイメージング学会理事・評議員。日本物理学会、応用物理学会、日本蛋白質科学会、日本生物物理学会、日本放射光学会会員

研究分野

1. 生体分子・分子集合体の動的構造と機能相関の研究
2. 蛋白質のフォールディング
3. X線顕微鏡の開発と細胞生物学への応用
4. バイオインフォーマティクスの臨床医学への応用
5. 食物アレルギー機構の分子科学的研究

著書

1. 木原 裕、菊地透：コメディカル物理学(開成出版)
2. 木原 裕：生命現象の物理(海文堂)
3. 木原 裕：わかりやすい物理学(ヌーベルヒロカワ)
4. 安岡 則武、木原 裕(編)：夢の光―放射光が拓く生命の神秘―(共立出版)

2014

1. Yi Shi, Ling Yu, Hiroshi Kihara and Jun-Mei Zhou (2014) C-terminal 13-residue truncation in-duces compact Trigger Factor conformation and severely impairs its dimerization ability, Protein & Peptide Letters, 21, 476-482.
2. Zhi-jie Qin, Akio Shimizu, Jinsong Li, Masamichi Ikeguchi, Masaji Shinjo and Hiroshi Kihara (2014) α-helix formation rate of loigopeptides at subzero temperatures, Biophysics, 10, 9-13.
3. Yoshitaka Matsumura, Xianju Jin, Jinsong Li, Masaji Shinjo, Kuniko Takemoto and Hiroshi Kihara (2014) Radiation protection effect of ethylene glycol and glycerol on proteins, J. Studies of Himeji-Hinomoto College, 36, 1-15.
4. Yi Shi, Masaji Shinjo, Jun-Mei Zhou and Hiroshi Kihara (2014) Structural stability of E. coli trigger factor studied by synchrotron small-angle X-ray scattering, Biophys. Chem., in press.

2013

1. Guryanov SG, Filimonov VV, Timchenko AA, Melnik BS, Kihara H, Kutyshenko VP, Ovchinnikov LP, Semisotnov GV. (2013) The major mRNP protein YB-1: structural and association properties in solution, 1834(2):559-567.
2. Timchenko AA, Novosylna OV, Prituzhalov EA, Kihara H, El'skaya AV, Negrutskii BS, Serdyuk IN. (2013) Different oligomeric properties and stability of highly homologous A1 and proto-oncogenic A2 variants of mammalian translation elongation factor eEF1,Biochemistry, 52(32):5345-5353.
3. Matsumura Y, Shinjo M, Kim SJ, Okishio N, Gruebele M, Kihara H. (2013) Transient helical structure during PI3K and Fyn SH3 domain folding, J Phys Chem B, 4836-4843.
4. Matsumura Y, Shinjo M, Matsui T, Ichimura K, Song J, Kihara H. (2013) Structural study of hNck2 SH3 domain protein in solution by circular dichroism and X-ray solution scattering, Biophys Chem.;175-176:39-46.
5. Masashi Yoshimura, Kuniko Takemoto, Takuji Ohigashi,Hiroshi Kihara, Hidetoshi Namba, Toshiaki Ohta (2013) 2D/3D elemental mapping using soft X-ray microscopy, Memoirs of the SR Center, Ritsumeikan University15:189-191
6. Kuniko Takemoto, Masashi Yoshimura2 Satoshi Ichise, Hidetoshi Namba and Hiroshi Kihara (2013) Soft X-ray Imaging of Microstructure of Phormidium tenue under Different Culture Conditions, Memoirs of the SR Center, Ritsumeikan University 15:187-188
7. 木原 裕、新庄 正路、松村 義隆 (2013) クライオストップトフローX線溶液散乱法を用いたタンパク質フォールディング初期中間体の構造解析、日本結晶学会誌 55, 271-277.
8. Yoshitaka Matsumura, Masaji Shinjo and Hiroshi Kihara, Effect of ethylene glycol on protein conformation, The Journal of the Department of Liberal Arts of Kansai Medical University, 33 (2013) 53-62
9. 木原 裕 (2013) 医科大学における教養の役割、関西医科大学教養部紀要、33, 17-18.

2012

1. Katina N, Timchenko A, Kihara H, Balobanov V, Vasiliev V, Kashparov I, Bychkova V (2012) Kinetics of mutant apomyoglobin association, Macromolecular symposia 317-318(1):215-226
2. K. Takemoto, H. Ikegaya, T. Yaji, S. Ichise, T. Ohta and H. Kihara (2012) Infrared Micro-Spectroscopy Observation of plantplankton with in Lake Biwa, Memoirs of the SR Center, Ritsumeikan University 14:173-174
3. K. Takemoto, G. Mizuta, M. Yoshimura, S. Ichise, H. Namba and H. Kihara (2012) Soft x-ray imaging of intracellular structure of Leptolyngbya tenuis (Phormidium tenue) generating musty smell in Lake Biwa, Memoirs of the SR Center, Ritsumeikan University 14:171-172
4. Takuji Ohigasi, Haruo Mizutani, Hidetoshi Namba, Kuniko Takemoto, and Hiroshi Kihara (2012) 3-Demensional Observation of Cerebral Cortex of Mouse of Using Full-field Imaging Soft X-ray Microscopy, Memoirs of the SR Center, Ritsumeikan University 14:169-170
5. 市村 薫、木原 裕 (2012) トランスフェリンとトランスフェリンレセプター, 関西医科大学教養部紀要 32:39-47
6. 竹本 邦子、山本 章嗣、水田 剛、一瀬 諭、吉村 真史、難波 秀利、木原 裕 (2012) 琵琶湖産の糸状カビ臭産生藍藻Phormidium tenueの細胞内微細構造観察：軟X線顕微鏡と透過型電子顕微鏡および低真空クライオ走査型電子顕微鏡を用いた比較観察、日本水処理生物学会誌48(4):157-163

2011

1. K. Takemoto, S. Ichise, T. Ohigashi, H. Namba and H. Kihara (2011) X-ray Imaging of Mucilaginous Sheath of Phytoplankton in Lake Biwa by Soft X-ray Microscope, AIP Conf. Proc. 1365, 373.
2. K. Takemoto, I. Narumi, K. Satoh, T. Ohigashi, H. Namba and H. Kihara (2011) New Approach for X-ray Microimaging of Live Cells in the Carbon Window at Subzero Temperatures with the Use of Antifreeze, AIP Conf. Proc. 1365, 415.
3. Matsumura, Y. and Kihara, H. (2011) Stopped flow test reaction with of 2, 6-dichloroindophenol sodium, n-hydrate (DCIP) reduction by ascorbic acid, J. Dept. Liber-al Arts Kansai Med. Univ., 31: 135-143.
4. Kano, F., Shinjo, M., Qin, Z.J., Li, J.S., Matsumura, Y., Shimizu, A., Teramoto, A., and Kihara, H. (2011) Denaturant-induced helix-coil transition of oligopeptides: theoretical and equilibrium studies of short oligopeptides C17 and AK16, Polymer J., 43(3): 293-300.
5. T. Ohigashi, H. Fujii, K. Usui, H. Namba, H. Mizutani, K. Takemoto and H. Kihara (2011) Development of Computer Tomography System for the Soft X‐ray Microscope at Ritsumeikan University, AIP Conf. Proc. 1365, 124.
6. Mitsuhiro Kimura, Kuniko Takemoto, Yoshio Ohashi, Takuji Ohigashi, Koji Nakani-shi, Hidetoshi Namba, Hiroshi Kihara (2011) Observation of Biospecimens at Cryotem-peratures with Soft X-ray Microscope, Jpn J Appl Phys 50(2), 026601-026601-5.

2010

1. Matsumura, Y., Shinjo, M., Mahajan, A., Tsai, M. D., Kihara, H. (2010) al-pha-Helical burst on the folding pathway of FHA domains from Rad53 and Ki67., Biochimie, 92(8): 1031-1039.
2. Kimura, M., Takemoto, K. , Kihara, H. (2010) Soft X-ray Microscope at Cryo Temper-ature,J. Dept. Liberal Arts Kansai Med. Univ., 30: 25-54.
3. Ohigashi, T. , Fujii, H., Usui, K., Namba, H.., Takemoto, K. and Kihara, H. (2010) Development of 3-dimensional imaging system for the full-field imaging soft X-ray microscope at BL-12, Memoirs of SR center, Ritsumeikan U., 12: 67-73.
4. Kimura, M., Takemoto, K., Ohashi, Y., Ohigashi, T., Nakanishi, K., Namba, H.., and Kihara, H. (2010) Soft X-ray microsopic image at cryo temperature, Memoirs of SR center, Ritsumeikan U., 12: 171-172.
5. Takemoto, K., Ichise, S., Ohigashi, T., Namba, H. and Kihara, H. (2010) X-ray imag-ing of mucilaginous sheath of phytoplankton in Lake Biwa by soft X-ray microscope, Memoirs of SR center, Ritsumeikan U., 12: 169-170.

2009

1. Kogo. H., Takeuchi,K., Inoue, H., Kihara, H., Kojima, M., Takahashi, K. (2009) Urea-dependent unfolding of HIV-1 protease studied by circular dichroism and small-angle X-ray scattering, Biochim Biophys Acta- Proteins and Proteomics, 1794: 70-74.
2. Kim, S.J., Matsumura, Y., Dumont, C,, Kihara, H., Gruebele, M. (2009) Slowing down downhill folding: a three-probe study, Biophys J., 97(1): 295-302.
3. Bellgrove, A,, Kihara, H., Iwata, A,, Aoki, M.N., Heraud, P. (2009) Fourier trans-form infrared microspectroscopy as a tool to identify macroalgal propagules, J. Phycol, 45(3): 560-570.
4. Yamamoto, A., Takemoto, K., Fukui, T., Yoshimura, Y., Okuno, K., Namba, H., Ki-hara, H., (2009) Observation of Immuno-Labeled Cells at High Resolution Using Soft X-Ray Microscope at Ritsumeikan University SR Center, Journal of Physics: Conference Series, 186: Article number 012099.
5. Yamamoto, A., Takemoto, K., Komura I, Namba Kihara, H. (2009) Imaging of Chromosomes at Nanometer-Scale Resolution Using Soft X-Ray Microscope at Ritsumeikan University SR Center, Journal of Physics: Conference Series, 186: Article number 012098.
6. Takemoto, K., Ichise S, Ichikawa M, Namba Kihara, H., H. (2009) X-ray imaging of Picoplankton in Lake Biwa by Soft X-ray Microscope at Ritsumeikan University SR Center, Journal of Physics: Conference Series, 186: Article number 012097.
7. Takemoto, K., Narumi I, Satoh K, Namba Kihara, H., H. (2009) X-Ray Imaging of Radioresistant Deinococcus radiodurans, Journal of Physics: Conference Series, 186: Article number 012096.
8. Takemoto, K., Namba, H., Mukai M, Okuno, K., Ohta T, Kihara, H. (2009) Development of an Auto-focusing Imaging System in the Soft X-Ray Microscope Beamline of the SR Center in Ritsumeikan University, Journal of Physics: Conference Series, 186: Article number 012019.
9. Ohigashi, T., Fujii H, Aratame Namba, H.. H, Takemoto, K., Kihara, H., Inaba M, Kubokawa K (2009) Anatomical Observation of a Lancelet by a Full-Field Imaging Soft X-ray Microscopy, Mem SR Cent Ritsumeikan Univ., (11): 155-157.
10. Takemoto, K., Kimura, M., Kihara, H., Ohigashi, T., Fujii H, Aratame H, Ohashi, Y., Nakanishi, K., Namba, H. (2009) Recent Progress in Transmission Soft X-ray Micro-scope, Mem. SR Cent Ritsumeikan Univ., (11): 141-149.
11. Tsukamoto S, Yamashita T, Yamada Y, Fujiwara K, Maki Kuwajima, K. K, Matsumura, Y., Kihara, H., Tsuge H, Ikeguchi M (2009) Non-native α-helix formation is not neccessary for folding of lipocalin: comparison of burst-phase folding intermediates between tear lipocalin and β-lactoglobulin, Proteins, 76(1): 226-236.
12. Tsukamoto, Seiichi, Yamashita, Takako, Yamada, Yoshiteru, Fujiwara, Kazuo, Maki, Kosuke, Kuwajima, Kunihiro, Matsumura, Yoshitaka, Kihara, Hiroshi, Tsuge, Hideaki, Ikeguchi, Masamichi (2009) Correction to the article "Non-native alpha-helix formation is not necessary for folding of lipocalin: Comparison of burst-phase folding between tear lipocalin and beta-lactoglobulin", Proteins.
13. B. Fayard, M. Salomé, K. Takemoto, H. Kihara, J. Susini (2009) Some practical considerations about the effects of radiation damage on hydrated cells imaged by X-ray fluorescence microscopy, Journal of Electron Spectroscopy and Related Phenomena, 170, 19–24.

2008

1. Tashiro M, Kojima, M., Kihara, H., Kasai, K., Kamiyoshihara, T., Uéda K, Shimo-takahara, S. (2008) Characterization of fibrillation process of alpha-synuclein at the in-itial stage, Biochem. Biophys. Res. Commun., 369(3): 910-914.
2. Prokhorov, D., Timchenko, A.A, Uversky, V.N., Khristoforov, V.S., Kihara, H., Ki-mura K, and Kutyshenko, V.P. (2008) Dynamics of oligomer formation by denatured carbonic anhydrase II, Biochim Biophys Acta, 1784(5): 834-842.
3. Matsumura, Y., Li. J.S., Ikeguchi, M., Kihara, H. (2008) Helix-rich transient and equilibrium intermediates of equine beta-lactoglobulin in alkaline buffer, Biophys. Chem., 134(1-2): 84-92.
4. Kojima, M., Kezuka, Y., Nonaka, T., Hiragi, Y., Watanabe, T., Kimura, K., Takahashi, K., Yanagi, S., and Kihara, H. (2008) SaxsMDView: A three-dimensional graphics program for displaying force vectors, J. Synchrotron Radiat., 15(5): 535-537.
5. Takemoto, K., Ichise S, Ichikawa M, Namba, H., and Kihara, H. (2008) X-ray Imaging of Picoplankton in Lake Biwa by Soft X-ray Microscope, Mem SR Cent Ritsumeikan Univ, 10: 168-169.
6. Kimura, M., Takemoto, K., Ohashi, Y., Nakanishi, K., Namba, H., and Kihara, H. (2008) Development of Cryogenic System for Soft X-ray Microscope, Mem. SR Center Ritsumeikan Univ., 10: 138-148.

2007

1. Takemoto, K., and Kihara, H. (2007) Feasibility study on X-ray microscopy in tu-mor diagnosis at sub-cellular level, AIP Conf. Proc., 902: 45-48.
2. Shinomiya, H., Shinjo, M., Fengzhi, L., Asano, Y, and Kihara, H. (2007) Conformational analysis of the leukocyte-specific EF-hand protein p65/L-plastin by X-ray scattering in solution, Biophys. Chem., 131(1-3): 36-42.
3. Timchenko, A.A., Shiryaev, V., Fedorova, Yu., Kihara, H., Kimura, K., Willumeit, R., Garamus, V., and Selivanova, O. (2007) Conformation of Thermus thermophilus ribosomal protein S1 in solution at different ionic strengths, Biophysics, 52(2): 162-167.
4. Yamada, Y., Yajima, T., Tsukamoto, S., Nakagawa, K., Fujiwara, K., Kihara, H., and Ikeguchi, M. (2007) Chloride-ion concentration dependence of molecular dimension in the acid-denatured state of equine β-lactoglobulin, J. Appl. Crystallogr., 40 (SUPPL. 1): s213-s216.
5. Takemoto, K., Yamamoto, A., Komura, I., Nakanishi, K., Namba, H., and Kihara, H. (2007) Imaging of Chromosomes at Nanometer-Scale Resolution, Using Soft X-Ray Microscope, Mem. SR Center Ritsumeikan Univ., 9: 85-89.
6. Kimura, M., Takemoto, K., and Kihara, H. (2007) Development of Cryogenic Sam-ple Chamber in Soft X-Ray Microscope at BL-12, Mem. SR Center Ritsumeikan Univ., 9: 79-84.
7. Li, J.S., Shinjo, M., Matsumura, Y., Morita, M., Baker, D., Ikeguchi, M., and Kiha-ra, H. (2007) An alpha-helical burst in the src SH3 folding pathway, Biochemistry, 46(17): 5072-5082.
8. Li, J.S., Matsumura, Y., Shinjo, M., Kojima, M., and Kihara, H. (2007) A stable alpha-helix-rich intermediate is formed by a single mutation of the beta-sheet protein, src SH3, at pH 3, J. Mol. Biol., 372(3): 747-755.

2006

1. Timchenko, A.A., Kubareva, E.A., Volkov, E.M., Voronina. O.L., Lunin. V.G., Gon-char. D.A., Degtyarev. S.Kh.、Timchenko. M.A., Kihara, H., Kimura, K. (2006) Struc-ture of Escherichia coli uracil-DNA glycosylase and its complexes with nonhydrolyzable substrate analogues in solution studied by synchrotron small-angle X-ray scattering, Biophysics (Oxf), 51(1): 1-7.
2. Qin, Z.J., Vyas, S.,. Fink, A.L., Li, J.S. and Kihara, H. (2006) Transient al-pha-helical structure during folding of Src SH3 domain at subzero temperatures, J. Kan-sai Med. Univ., 58: 163-169.
3. Mukai, M., Yamamoto, A., Takemoto, K., Okuno, K., Okamoto T, Oohata, A., and Kihara, H. (2006) X-Ray Micro-imag of Vanadium Uptake in Dictyostelium discoideum at BL12, Mem. SR Center Ritsumeikan Univ., 8: 159-165.
4. Yamamoto, A., Fukui Y, Yoshimura, Y., Okuno, K., Takemoto, K., Okamoto, T., Namba, H., Kihara, H. (2006) Observation of Immuno-Labeled Cells at High Resolution Using Soft X-Ray Microscopy, Mem. SR Center Ritsumeikan Univ., 8: 151-157.
5. Takemoto, K., Okuno, K., Fukui K, Yoshimura, Y., Mukai, M., Yamamoto, A., Oka-moto, T., Namba, H., and Kihara, H. (2006) Recent Observation of Biospecimens by Soft X-Ray Microscope BL12, Mem. SR Center Ritsumeikan Univ., 8: 143-149.

2005

1. Yamada, Y., Yajima, T., Fujiwara, K., Arai, M., Ito, K., Shimizu, A., Kihara, H., Kuwajima, K., Amemiya, Y., Ikeguchi, M. (2005) Helical and expanded conformation of equine beta-lactoglobulin in the cold-denatured state, J. Mol. Biol., 350(2): 338-348.
2. Kimura, M., Takemoto, K., Kihara, H. (2005) Development of auto focusing system of soft X-ray microscope, Mem. SR Center Ritsumeikan Univ., 7: 77-82.
3. Takemoto, K., Okuno, K., Okamoto, T., Kubo, M., Kihara, H. (2005) Quanti-tative evaluation of transmission of x-ray micrograph, Mem. SR Center Ritsumeikan Univ., 7: 113-114.